Peptide sequencing is a vital process in biochemistry and molecular biology that involves determining the precise order of amino acids in a peptides. Peptides hold shorter chains of amino acids than proteins, and play crucial roles in biological processes and can function as hormones, neurotransmitters, and immune system components. The sequence of a peptide is critical to understanding its structure, function, and interactions with other molecules. Modern peptide sequencing techniques often employ mass spectrometry and Edman degradation, which can accurately identify the amino acids and their order in the peptide. Mass spectrometry, in particular, has become increasingly popular due to its sensitivity and ability to analyze complex mixtures of peptides. Through peptide sequencing, scientists can gain insights into protein function and regulation, aiding in developing new therapeutics and understanding various biological processes.